pentr d topo vector backbone Search Results


pentr vector intermediate  (Addgene inc)
96
Addgene inc pentr vector intermediate
Pentr Vector Intermediate, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+d+topo+vector+backbone/pcDNA3%2E1(%2B)+CircRNA+Mini+Vector+(Plasmid+%2360648)/bio_rxiv__137307-271-13-30
Average 96 stars, based on 1 article reviews
pentr vector intermediate - by Bioz Stars, 2026-09
96/100 stars
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pentr vector  (Addgene inc)
96
Addgene inc pentr vector
<t>SUMO1P3</t> depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and <t>pENTR</t> (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.
Pentr Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+d+topo+vector+backbone/pcDNA3%2E1(%2B)+Laccase2+MCS+Exon+Vector+(Plasmid+%2369893)/pmc08148505-155-21-23
Average 96 stars, based on 1 article reviews
pentr vector - by Bioz Stars, 2026-09
96/100 stars
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entry vector  (IBA Lifesciences)
90
IBA Lifesciences entry vector
<t>SUMO1P3</t> depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and <t>pENTR</t> (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.
Entry Vector, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+d+topo+vector+backbone/pENTRY-IBA51+vector/pm39858499-37-19-22
Average 90 stars, based on 1 article reviews
entry vector - by Bioz Stars, 2026-09
90/100 stars
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h1 aavs1 cag gfp  (Addgene inc)
90
Addgene inc h1 aavs1 cag gfp
<t>SUMO1P3</t> depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and <t>pENTR</t> (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.
H1 Aavs1 Cag Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+d+topo+vector+backbone/pENTR%3A3xHAgCYCLOPS-S14A+(Plasmid+%2398045)/pm30799279-376-93-111
Average 90 stars, based on 1 article reviews
h1 aavs1 cag gfp - by Bioz Stars, 2026-09
90/100 stars
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entry vector pentrppu6p sgrna l1l2  (Addgene inc)
93
Addgene inc entry vector pentrppu6p sgrna l1l2
<t>SUMO1P3</t> depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and <t>pENTR</t> (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.
Entry Vector Pentrppu6p Sgrna L1l2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+d+topo+vector+backbone/pENTR-PpU6P-sgRNA-L1L2+(Plasmid+%23113735)/pmc08320657-40-23-31
Average 93 stars, based on 1 article reviews
entry vector pentrppu6p sgrna l1l2 - by Bioz Stars, 2026-09
93/100 stars
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promoters recombinant dna reagent pentr d topo  (Thermo Fisher)
99
Thermo Fisher promoters recombinant dna reagent pentr d topo
<t>SUMO1P3</t> depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and <t>pENTR</t> (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.
Promoters Recombinant Dna Reagent Pentr D Topo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+d+topo+vector+backbone/DNA/10__7554_slash_elife__47864-450-142-148
Average 99 stars, based on 1 article reviews
promoters recombinant dna reagent pentr d topo - by Bioz Stars, 2026-09
99/100 stars
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pentr zeo vector  (Thermo Fisher)
99
Thermo Fisher pentr zeo vector
<t>SUMO1P3</t> depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and <t>pENTR</t> (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.
Pentr Zeo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+d+topo+vector+backbone/Zeocin/bio_rxiv__2020__07__28__222919-247-30-36
Average 99 stars, based on 1 article reviews
pentr zeo vector - by Bioz Stars, 2026-09
99/100 stars
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pentr™/u6 rnai entry vector  (Promega)
90
Promega pentr™/u6 rnai entry vector
<t>SUMO1P3</t> depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and <t>pENTR</t> (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.
Pentr™/U6 Rnai Entry Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+d+topo+vector+backbone/pentr++u6+rnai+entry+vector/pm31816429-53-6-22
Average 90 stars, based on 1 article reviews
pentr™/u6 rnai entry vector - by Bioz Stars, 2026-09
90/100 stars
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pentr4 vector  (Addgene inc)
92
Addgene inc pentr4 vector
<t>SUMO1P3</t> depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and <t>pENTR</t> (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.
Pentr4 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+d+topo+vector+backbone/pENTR4-V5+(w71-3)+(Plasmid+%2317425)/bio_rxiv__2024__11__25__625320-169-10-12
Average 92 stars, based on 1 article reviews
pentr4 vector - by Bioz Stars, 2026-09
92/100 stars
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creert2  (Addgene inc)
93
Addgene inc creert2
<t>SUMO1P3</t> depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and <t>pENTR</t> (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.
Creert2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+d+topo+vector+backbone/pENTR-CreERT2+(Plasmid+%23139547)/pm40135929-172-13-16
Average 93 stars, based on 1 article reviews
creert2 - by Bioz Stars, 2026-09
93/100 stars
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pentr psuper entry vector  (Addgene inc)
93
Addgene inc pentr psuper entry vector
<t>SUMO1P3</t> depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and <t>pENTR</t> (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.
Pentr Psuper Entry Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+d+topo+vector+backbone/pENTR%2FpSUPER%2B+(575-1)+(Plasmid+%2317338)/bio_rxiv__2020__06__15__152942-54-59-63
Average 93 stars, based on 1 article reviews
pentr psuper entry vector - by Bioz Stars, 2026-09
93/100 stars
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entry vector pentr5  (Addgene inc)
92
Addgene inc entry vector pentr5
<t>SUMO1P3</t> depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and <t>pENTR</t> (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.
Entry Vector Pentr5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+d+topo+vector+backbone/pFCK(1%2E3)GW+(Plasmid+%2327230)/bio_rxiv__2021__04__14__439926-110-0-5
Average 92 stars, based on 1 article reviews
entry vector pentr5 - by Bioz Stars, 2026-09
92/100 stars
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Image Search Results


SUMO1P3 depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and pENTR (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.

Journal: Aging (Albany NY)

Article Title: LncRNA SUMO1P3 acts as a prognostic biomarker and promotes hepatocellular carcinoma growth and metastasis

doi: 10.18632/aging.202921

Figure Lengend Snippet: SUMO1P3 depletion impeded HCC growth and metastasis in vivo . Four-week-old male SCID mice were inoculated subcutaneously into the hind flanks or injected via tail vein with MHCC97H-luc cells with stably expressing shNC or shSUMO1P3-2. ( A ) Tumor volumes were monitored and calculated every week for 6 weeks. ( B ) Representative images of the mice (Mock and pENTR (n=10), or pENTR-shSUMO1P3 and pENTR-shSUMO1P3 (n=10)) photographed with an IVIS Imaging System at days 14 after inoculation. ( C ) Representative general photographs and HE staining assay of the harvested primary tumors at 6 weeks after subcutaneous xenografting. 100 × magnification in HE sections. ( D ) TUNEL assay was carried out to determine cell apoptosis in the tumor tissues of the xenografts. The percentage of TUNEL-positive cells was calculated. ( E , F ) At 8 weeks after injection through the tail vein, the lungs were harvested and photographed, and the numbers of pulmonary metastatic nodules was counted. ( G ) SUMO1P3 expression in tumor tissues was measured via qPCR assay. GAPDH was used as the endogenous control. ( H ) Representative results of Western blot analyses of cyclin D1, p-Akt, Akt, Bax, Bcl-2, cl-caspase-3, caspase-3, E-cadherin, vimentin, MMP-2, and MMP-9 in tumor tissues. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Mock or pENTR group.

Article Snippet: For lentiviral construction, the sequence of short hairpin RNA (shRNA) targeting SUMO1P3 (shSUMO1P3) or a scrambled shRNA (shNC) was cloned into pENTR vector (Addgene, Cambridge, MA, USA). pMDLg/pRRE and pRSV-REV are lentiviral packaging plasmids.

Techniques: In Vivo, Injection, Stable Transfection, Expressing, Imaging, Staining, TUNEL Assay, Western Blot